Changes in chloride homeostasis-regulating gene expressions in the rat hippocampus following amygdala kindling

Brain Res. 2003 Nov 14;990(1-2):221-6. doi: 10.1016/s0006-8993(03)03528-5.

Abstract

In a rat kindling model, we examined expression patterns of NKCC1, KCC1, KCC2, and CLC-2. In the dentate granule cell layer, there was an activity-dependent increase in NKCC1 mRNA but significant decreases in KCC1 and CLC-2 mRNAs. In addition, CLC-2 mRNA expression was markedly decreased in CA1 pyramidal layer. These results suggest that an increase in [Cl-]i and a resultant reduction in GABAergic inhibition may occur in hippocampus of epileptic rats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amygdala / physiology*
  • Animals
  • Autoradiography
  • Chloride Channels / biosynthesis
  • Chloride Channels / genetics
  • Chlorides / metabolism*
  • Gene Expression Regulation / physiology*
  • Hippocampus / metabolism*
  • Homeostasis / genetics*
  • Kindling, Neurologic / physiology*
  • Male
  • Pyramidal Cells / metabolism
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Rats
  • Rats, Wistar
  • Sodium-Potassium-Chloride Symporters / biosynthesis
  • Sodium-Potassium-Chloride Symporters / genetics
  • Solute Carrier Family 12, Member 2
  • Symporters / biosynthesis
  • Symporters / genetics
  • gamma-Aminobutyric Acid / physiology

Substances

  • Chloride Channels
  • Chlorides
  • ClC-2 chloride channels
  • RNA, Messenger
  • Slc12a2 protein, rat
  • Sodium-Potassium-Chloride Symporters
  • Solute Carrier Family 12, Member 2
  • Symporters
  • potassium-chloride symporters
  • gamma-Aminobutyric Acid