Ospdr9, which encodes a PDR-type ABC transporter, is induced by heavy metals, hypoxic stress and redox perturbations in rice roots

FEBS Lett. 2003 Oct 23;553(3):370-6. doi: 10.1016/s0014-5793(03)01060-3.

Abstract

Little is known about the role of pleiotropic drug resistance (PDR)-type ATP-binding (ABC) proteins in plant responses to environmental stresses. We characterised ospdr9, which encodes a rice ABC protein with a reverse (ABC-TMS(6))(2) configuration. Polyethylene glycol and the heavy metals Cd (20 microM) and Zn (30 microM) rapidly and markedly induced ospdr9 in roots of rice seedlings. Hypoxic stress also induced ospdr9 in rice roots, salt stress induced ospdr9 at low levels but cold and heat shock had no effect. The plant growth regulator jasmonic acid, the auxin alpha-naphthalene acetic acid and the cytokinin 6-benzylaminopurine triggered ospdr9 expression. The antioxidants dithiothreitol and ascorbic acid rapidly and markedly induced ospdr9 in rice roots; the strong oxidant hydrogen peroxide also induced ospdr9 but at three times lower levels. The results suggested that redox changes may be involved in the abiotic stress response regulation of ospdr9 in rice roots.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / drug effects
  • ATP-Binding Cassette Transporters / genetics*
  • ATP-Binding Cassette Transporters / metabolism*
  • Ascorbic Acid / pharmacology
  • Cadmium / pharmacology*
  • Dithiothreitol / pharmacology
  • Drug Resistance, Multiple / genetics*
  • Gene Expression Regulation, Plant / physiology*
  • Hydrogen Peroxide / pharmacology
  • Hypoxia / metabolism*
  • Oryza / drug effects
  • Oryza / genetics*
  • Oryza / metabolism*
  • Oxidation-Reduction
  • Plant Growth Regulators / pharmacology
  • Plant Roots / drug effects
  • Plant Roots / genetics
  • Plant Roots / metabolism
  • Polyethylene Glycols / pharmacology
  • RNA, Plant / analysis
  • Seedlings / drug effects
  • Seedlings / metabolism
  • Sodium Chloride / pharmacology
  • Temperature
  • Zinc / pharmacology*
  • Zinc / toxicity
  • rac1 GTP-Binding Protein / biosynthesis

Substances

  • ATP-Binding Cassette Transporters
  • Plant Growth Regulators
  • RNA, Plant
  • Cadmium
  • Polyethylene Glycols
  • Sodium Chloride
  • Hydrogen Peroxide
  • rac1 GTP-Binding Protein
  • Zinc
  • Ascorbic Acid
  • Dithiothreitol