Lentin, a novel and potent antifungal protein from shitake mushroom with inhibitory effects on activity of human immunodeficiency virus-1 reverse transcriptase and proliferation of leukemia cells

Life Sci. 2003 Nov 14;73(26):3363-74. doi: 10.1016/j.lfs.2003.06.023.


From the fruiting bodies of the edible mushroom Lentinus edodes, a novel protein designated lentin with potent antifungal activity was isolated. Lentin was unadsorbed on DEAE-cellulose, and adsorbed on Affi-gel blue gel and Mono S. The N-terminal sequence of lentin manifested similarity to endoglucanase. Lentin, which had a molecular mass of 27.5 kDa, inhibited mycelial growth in a variety of fungal species including Physalospora piricola, Botrytis cinerea and Mycosphaerella arachidicola. Lentin also exerted an inhibitory activity on HIV-1 reverse transcriptase and proliferation of leukemia cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antifungal Agents / pharmacology*
  • Cell Division / drug effects
  • Cell Line, Tumor / drug effects
  • Fungal Proteins / pharmacology*
  • HIV Reverse Transcriptase / antagonists & inhibitors*
  • Leukemia / drug therapy
  • Plant Extracts / pharmacology*
  • Rabbits
  • Reverse Transcriptase Inhibitors / pharmacology*
  • Shiitake Mushrooms / chemistry*


  • Antifungal Agents
  • Fungal Proteins
  • Plant Extracts
  • Reverse Transcriptase Inhibitors
  • lentin protein, Lentinus edodes
  • HIV Reverse Transcriptase