Expression of the oligodendroglial lineage-associated markers Olig1 and Olig2 in different types of human gliomas

J Neuropathol Exp Neurol. 2003 Oct;62(10):1052-9. doi: 10.1093/jnen/62.10.1052.


Because a specific group of oligodendrogliomas is susceptible to adjuvant therapy, it is important to elucidate the biological characteristics of these tumors. In situ hybridization analyses have revealed that Olig genes are expressed in oligodendroglial lineage cells and are highly expressed in oligodendrogliomas. To clarify whether OLIG is a tumor-specific marker for oligodendrogliomas, we have investigated the expression of Olig transcripts by semiquantitative RT-PCR assay and OLIG2 protein with a new antibody in a variety of glial tumors. The semiquantitative RT-PCR revealed that high levels of expression of Olig1 and Olig2 mRNAs were present in anaplastic oligodendrogliomas and anaplastic astrocytomas, while expression of these mRNAs in grade IV glioblastomas was lower than in grade II and grade III gliomas (p < 0.01). Immunohistochemical analyses demonstrated that the mean immunopositive proportion of OLIG2 was 82% in anaplastic oligodendrogliomas but only 34% in anaplastic astrocytomas. Therefore, although OLIG2 expression was detected in a range of gliomas not specific for oligodendrogliomas, the expression level in anaplastic oligodendrogliomas was more uniform and intense than that in other glial tumors. In conclusion, combining Olig mRNA expression and immunohistochemistry of OLIG2 enables oligodendrogliomas to be distinguished from glioblastomas and other astrocytic glial tumors.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adrenal Gland Neoplasms
  • Adult
  • Aged
  • Aging
  • Basic Helix-Loop-Helix Transcription Factors
  • Brain / anatomy & histology
  • Brain / metabolism
  • Brain / pathology
  • Cell Count
  • Child
  • Child, Preschool
  • DNA-Binding Proteins*
  • Ganglion Cysts / metabolism
  • Glioblastoma / classification
  • Glioblastoma / genetics
  • Glioblastoma / metabolism*
  • Humans
  • Immunoblotting
  • Immunohistochemistry
  • Infant
  • Middle Aged
  • Nerve Tissue Proteins / biosynthesis*
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / immunology
  • Oligodendrocyte Transcription Factor 2
  • RNA, Messenger / biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Cells, Cultured


  • Basic Helix-Loop-Helix Transcription Factors
  • DNA-Binding Proteins
  • Nerve Tissue Proteins
  • OLIG1 protein, human
  • OLIG2 protein, human
  • Oligodendrocyte Transcription Factor 2
  • RNA, Messenger