Expression of bacterial beta-galactosidase in animal cells

G An; K Hidaka; L Siminovitch

doi:10.1128/mcb.2.12.1628-1632.1982

Expression of bacterial beta-galactosidase in animal cells

Mol Cell Biol. 1982 Dec;2(12):1628-32. doi: 10.1128/mcb.2.12.1628-1632.1982.

Authors

G An¹, K Hidaka, L Siminovitch

Affiliation

¹ Department of Medical Genetics, University of Toronto, Hospital for Sick Children, Toronto, Ontario, Canada.

Abstract

A recombinant plasmid containing the gene for bacterial beta-galactosidase, situated close to the simian virus 40 early promoter, has been constructed. Transfection of CHO, L, and COS-1 cells with this plasmid led to the expression and appearance of the enzyme. Using this system, we have developed a series of promoter cloning vehicles capable of accepting promoter signals for animal genes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Proteins / biosynthesis*
Bacterial Proteins / genetics
Cell Line
Escherichia coli / genetics
Gene Expression Regulation
Genes, Bacterial
Plasmids / genetics
Plasmids / metabolism
Promoter Regions, Genetic
Recombinant Proteins / biosynthesis
beta-Galactosidase / biosynthesis*
beta-Galactosidase / genetics

Substances

Bacterial Proteins
Recombinant Proteins
beta-Galactosidase