Isolation of Putrescine-Requiring Mutants of Neurospora Crassa

Antonie Van Leeuwenhoek. 1977;43(2):143-51. doi: 10.1007/BF00395669.

Abstract

Two auxotrophs of Neurospora crassa have been isolated that give a positive growth response to putrescine, spermidine or spermine. One of the mutants is deficient in ornithine decarboxylase activity and has been designated put-I. Both mutants map on linkage group VR, fail to complement and are infertile when crossed to one another, indicating that they are probably alleles. A putrescine auxotroph is incapable of suppressing a pro-4 mutant. The isolation of the mutants confirms that putrescine is an essential factor the normal growth of the organism, and is synthesized via a single pathway in Neurospora.

MeSH terms

  • Crosses, Genetic
  • Genetic Complementation Test
  • Genetic Linkage
  • Mutation
  • Neurospora / metabolism*
  • Neurospora crassa / genetics
  • Neurospora crassa / metabolism*
  • Ornithine Decarboxylase / metabolism
  • Putrescine / metabolism*
  • Spermidine / metabolism
  • Spermine / metabolism

Substances

  • Spermine
  • Ornithine Decarboxylase
  • Spermidine
  • Putrescine