Addition of a peptide tag at the C terminus of AtHKT1 inhibits its Na+ transport

Biosci Biotechnol Biochem. 2003 Oct;67(10):2291-3. doi: 10.1271/bbb.67.2291.

Abstract

Eight transmembrane segments in the Arabidopsis Na+ transporter, AtHKT1, have been proposed to be arranged around the axis of the pore. We report here that the addition of a GFP or a FLAG tag to the C terminus of AtHKT1 impairs the Na+ transport in Xenopus ooytes as detected by electrophysiological measurements.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arabidopsis Proteins / antagonists & inhibitors
  • Arabidopsis Proteins / chemistry
  • Arabidopsis Proteins / metabolism*
  • Cation Transport Proteins / antagonists & inhibitors
  • Cation Transport Proteins / chemistry
  • Cation Transport Proteins / metabolism*
  • Electrophysiology
  • Green Fluorescent Proteins
  • Luminescent Proteins / pharmacology
  • Molecular Probes / pharmacology*
  • Oligopeptides
  • Oocytes
  • Peptides / pharmacology
  • Plant Proteins*
  • Sodium / metabolism
  • Symporters*
  • Xenopus

Substances

  • Arabidopsis Proteins
  • Cation Transport Proteins
  • HKT1 protein, Arabidopsis
  • HKT1 protein, plant
  • Luminescent Proteins
  • Molecular Probes
  • Oligopeptides
  • Peptides
  • Plant Proteins
  • Symporters
  • Green Fluorescent Proteins
  • FLAG peptide
  • Sodium