Abstract
Eight transmembrane segments in the Arabidopsis Na+ transporter, AtHKT1, have been proposed to be arranged around the axis of the pore. We report here that the addition of a GFP or a FLAG tag to the C terminus of AtHKT1 impairs the Na+ transport in Xenopus ooytes as detected by electrophysiological measurements.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Arabidopsis Proteins / antagonists & inhibitors
-
Arabidopsis Proteins / chemistry
-
Arabidopsis Proteins / metabolism*
-
Cation Transport Proteins / antagonists & inhibitors
-
Cation Transport Proteins / chemistry
-
Cation Transport Proteins / metabolism*
-
Electrophysiology
-
Green Fluorescent Proteins
-
Luminescent Proteins / pharmacology
-
Molecular Probes / pharmacology*
-
Oligopeptides
-
Oocytes
-
Peptides / pharmacology
-
Plant Proteins*
-
Sodium / metabolism
-
Symporters*
-
Xenopus
Substances
-
Arabidopsis Proteins
-
Cation Transport Proteins
-
HKT1 protein, Arabidopsis
-
HKT1 protein, plant
-
Luminescent Proteins
-
Molecular Probes
-
Oligopeptides
-
Peptides
-
Plant Proteins
-
Symporters
-
Green Fluorescent Proteins
-
FLAG peptide
-
Sodium