Selective inhibition of cyclooxygenase 2 induces p27kip1 and skp2 in oral squamous cell carcinoma

J Otolaryngol. 2003 Aug;32(4):226-9. doi: 10.2310/7070.2003.41701.

Abstract

It has been shown that inhibition of cyclooxygenase 2 (COX-2) may cause growth arrest and reduced tumour formation in some human cancers; however, the mechanism is not fully known. In this study, we used an oral squamous cell carcinoma cell line to study growth inhibition and changes in critical cell cycle-regulating proteins induced by the selective COX-2 inhibitor celecoxib. Using cell viability assay, we established the optimal in vitro inhibitory dose of celecoxib and showed that inhibition of COX-2 markedly induces the expression of p27kip1, p21, waf1, and the F-box protein skp2. These results add new experimental data to our knowledge of the mechanism of cyclooxygenases on neoplastic cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinoma, Squamous Cell / drug therapy*
  • Carcinoma, Squamous Cell / metabolism
  • Celecoxib
  • Cell Cycle / drug effects
  • Cell Cycle Proteins / biosynthesis*
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Cyclin-Dependent Kinase Inhibitor p27
  • Cyclooxygenase 2
  • Cyclooxygenase 2 Inhibitors
  • Cyclooxygenase Inhibitors / pharmacology*
  • Cyclooxygenase Inhibitors / therapeutic use
  • Gene Expression Regulation, Neoplastic / drug effects
  • Humans
  • In Vitro Techniques
  • Indomethacin / pharmacology
  • Indomethacin / therapeutic use
  • Isoenzymes / antagonists & inhibitors*
  • Membrane Proteins
  • Mouth Neoplasms / drug therapy*
  • Mouth Neoplasms / metabolism
  • Prostaglandin-Endoperoxide Synthases
  • Pyrazoles
  • S-Phase Kinase-Associated Proteins / biosynthesis*
  • Sulfonamides / pharmacology
  • Sulfonamides / therapeutic use
  • Tumor Suppressor Protein p53 / biosynthesis
  • Tumor Suppressor Proteins / biosynthesis*

Substances

  • Cell Cycle Proteins
  • Cyclooxygenase 2 Inhibitors
  • Cyclooxygenase Inhibitors
  • Isoenzymes
  • Membrane Proteins
  • Pyrazoles
  • S-Phase Kinase-Associated Proteins
  • Sulfonamides
  • Tumor Suppressor Protein p53
  • Tumor Suppressor Proteins
  • Cyclin-Dependent Kinase Inhibitor p27
  • Cyclooxygenase 2
  • PTGS2 protein, human
  • Prostaglandin-Endoperoxide Synthases
  • Celecoxib
  • Indomethacin