High-resolution optical coherence microscopy for high-speed, in vivo cellular imaging

Opt Lett. 2003 Nov 1;28(21):2064-6. doi: 10.1364/ol.28.002064.

Abstract

Optical coherence microscopy (OCM) is demonstrated with a high-speed, broadband, reflective-grating phase modulator and a femtosecond Ti:Al2O3 laser. The novel system design permits high-resolution OCM imaging in a new operating regime in which a short coherence gate is used to relax the requirement for high-numerical-aperture confocal axial sectioning. In vivo cellular imaging is demonstrated in the Xenopus laevis tadpole and in human skin with a 3-microm coherence gate and a 30-microm confocal gate. The ability to achieve cellular imaging with a lower numerical aperture should facilitate the development of miniaturized probes for in vivo imaging applications.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cells / cytology*
  • Equipment Design
  • Humans
  • Microscopy, Confocal* / instrumentation
  • Skin / cytology
  • Tomography, Optical Coherence* / instrumentation
  • Xenopus laevis