Synthesis of linked carbohydrates and evaluation of their binding for 16S RNA by mass spectrometry

Baogen Wu; Jun Yang; Dale Robinson; Steve Hofstadler; Rich Griffey; Eric E Swayze; Yun He

doi:10.1016/j.bmcl.2003.09.005

Synthesis of linked carbohydrates and evaluation of their binding for 16S RNA by mass spectrometry

Bioorg Med Chem Lett. 2003 Nov 17;13(22):3915-8. doi: 10.1016/j.bmcl.2003.09.005.

Authors

Baogen Wu¹, Jun Yang, Dale Robinson, Steve Hofstadler, Rich Griffey, Eric E Swayze, Yun He

Affiliation

¹ Ibis Therapeutics, a division of Isis Pharmaceuticals, Inc., 2292 Faraday Avenue, Carlsbad, CA 92008, USA.

PMID: 14592474
DOI: 10.1016/j.bmcl.2003.09.005

Abstract

A library of linked molecules were synthesized from the common sugar moieties existing in the natural amino glycosides. These linked molecules were screened against bacterial 16S RNA for their binding affinity using a mass spectrometry-based technology. Some of these compounds exhibited low micromolar affinity and could serve as leads for further development as antibacterial agents.

MeSH terms

Base Sequence
Binding Sites
Carbohydrate Conformation
Carbohydrate Sequence
Escherichia coli / genetics
Mass Spectrometry
Molecular Sequence Data
Nucleic Acid Conformation
Oligosaccharides / chemical synthesis*
Oligosaccharides / chemistry*
RNA, Bacterial / chemistry
RNA, Ribosomal, 16S / chemistry*

Substances

Oligosaccharides
RNA, Bacterial
RNA, Ribosomal, 16S