An efficient enzymatic synthesis of thiamin pyrophosphate

Bioorg Med Chem Lett. 2003 Nov 17;13(22):4139-41. doi: 10.1016/j.bmcl.2003.07.026.

Abstract

Thiamin pyrophosphate was synthesized in 71% yield, on a multi-milligram scale, using overexpressed thiazole kinase, pyrimidine kinase, thiamin phosphate synthase, and thiamin phosphate kinase. This provides a facile route to isotopically labeled thiamin pyrophosphate from its readily available pyrimidine and thiazole precursors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • DNA Primers
  • Escherichia coli / enzymology
  • Phosphotransferases (Phosphate Group Acceptor)*
  • Polymerase Chain Reaction
  • Recombinant Proteins
  • Thiamine Pyrophosphate / chemical synthesis*

Substances

  • DNA Primers
  • Recombinant Proteins
  • Phosphotransferases (Phosphate Group Acceptor)
  • thiamin-phosphate kinase
  • Thiamine Pyrophosphate