Chased PCR: A modified inverse PCR technique to characterize flanking regions of AT-rich DNA fragments

J Mol Microbiol Biotechnol. 2003;6(1):1-5. doi: 10.1159/000073402.


The chased polymerase chain reaction (PCR) technique described here is a convenient method enabling the characterization of flanking regions of a known A/T-rich DNA fragment in two different successive steps. The first step includes a modified inverse PCR with inverted tail-to-tail primers, each with 35 overhanging nucleotides for the insertion into a carrier plasmid. The second step consists of a technique similar to a site-directed mutagenesis. The chased PCR technique is simple, quick, versatile and efficient; it improves the inverse PCR technique and may be applied to any ligation-linker method. Consequently, the techniques for PCR-based gene isolation are more suitable for the isolation of missing sequences of A/T-rich or complex DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Composition
  • Base Sequence
  • DNA Primers / genetics
  • DNA, Protozoan / chemistry*
  • DNA, Protozoan / genetics*
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Plasmids / genetics
  • Plasmodium falciparum / genetics*
  • Polymerase Chain Reaction / methods*


  • DNA Primers
  • DNA, Protozoan