Changes in some enzymes of microbodies and plastid development in excised radish cotyledons: effect of narciclasine

J Plant Physiol. 2003 Sep;160(9):1041-9. doi: 10.1078/0176-1617-00911.

Abstract

Narciclasine (NCS), isolated from mucilage of Narcissus bulb, showed inhibitory effects on growth and plastid development of excised radish cotyledons. NCS (0.1 mumol/L) started to show inhibitory effects on isocitrate lyase and hydroxypyruvate reductase activities after 24 h incubation in light. When NCS concentration was increased to 10 mumol/L, the activities of both enzymes are completely inhibited. From ultrastructural studies, NCS markedly prevented the degradation of protein bodies and lipid bodies, as well as chloroplast formation of excised radish cotyledons. There was only little degradation of protein and lipid bodies, and almost no chloroplast formation in the excised radish cotyledon treated with 1 mumol/L NCS. Therefore, our results provide clear evidence that NCS inhibited the transition of glyoxysomes and peroxisomes, and chloroplast development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Oxidoreductases / antagonists & inhibitors
  • Alkaloids / isolation & purification
  • Alkaloids / pharmacology*
  • Amaryllidaceae Alkaloids*
  • Cotyledon / drug effects
  • Cotyledon / enzymology
  • Enzyme Inhibitors / isolation & purification
  • Enzyme Inhibitors / pharmacology
  • Hydroxypyruvate Reductase
  • Isocitrate Lyase / antagonists & inhibitors
  • Microbodies / drug effects
  • Microbodies / enzymology
  • Microscopy, Electron
  • Narcissus / chemistry
  • Phenanthridines*
  • Plant Growth Regulators / isolation & purification
  • Plant Growth Regulators / pharmacology
  • Plastids / drug effects
  • Plastids / enzymology
  • Raphanus / drug effects*
  • Raphanus / enzymology*
  • Raphanus / ultrastructure

Substances

  • Alkaloids
  • Amaryllidaceae Alkaloids
  • Enzyme Inhibitors
  • Phenanthridines
  • Plant Growth Regulators
  • narciclasine
  • Alcohol Oxidoreductases
  • Hydroxypyruvate Reductase
  • Isocitrate Lyase