In vivo effect of mutations at the PRPP binding site of the Bacillus subtilis purine repressor

Pekka Rappu; Terhi Pullinen; Pekka Mäntsälä

doi:10.1128/JB.185.22.6728-6731.2003

In vivo effect of mutations at the PRPP binding site of the Bacillus subtilis purine repressor

J Bacteriol. 2003 Nov;185(22):6728-31. doi: 10.1128/JB.185.22.6728-6731.2003.

Authors

Pekka Rappu¹, Terhi Pullinen, Pekka Mäntsälä

Affiliation

¹ Department of Biochemistry and Food Chemistry, University of Turku, FIN-20014 Turku, Finland. pekka.rappu@utu.fi

Abstract

The Bacillus subtilis PurR mediates adenine repression and guanosine induction of purA. PRPP inhibits binding of PurR to DNA in vitro. Mutations in the PRPP binding motif of PurR caused strong repression regardless of purine exclusions or additions, establishing the role of PRPP as regulator of PurR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus subtilis / genetics
Bacillus subtilis / growth & development*
Bacillus subtilis / metabolism
Bacterial Proteins / chemistry
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Binding Sites
Gene Expression Regulation, Bacterial*
Mutation*
Phosphoribosyl Pyrophosphate / chemistry
Phosphoribosyl Pyrophosphate / metabolism*
Repressor Proteins / chemistry
Repressor Proteins / genetics*
Repressor Proteins / metabolism

Substances

Bacterial Proteins
PurR protein, Bacteria
Repressor Proteins
Phosphoribosyl Pyrophosphate