Conserved SNH domain of the proto-oncoprotein SYT interacts with components of the human chromatin remodelling complexes, while the QPGY repeat domain forms homo-oligomers

Oncogene. 2003 Nov 6;22(50):8156-67. doi: 10.1038/sj.onc.1207031.


Many studies have now established that the SWI/SNF chromatin remodelling complexes are involved in activation and repression of a variety of genes. In mammalian cells, these complexes contain the BRM and BRG1 helicase-like proteins that are thought to be responsible for nucleosome remodelling. The proto-oncoprotein SYT, involved in the unique translocation t(X;18) found in synovial sarcoma, is known to interact with human BRM (hBRM), thus providing a link between chromatin remodelling factors and human cancer. In this work, we address how SYT interacts with hBRM and BRG1. We demonstrate that the conserved N-terminal SNH domain of SYT, which is also present in the oncoproteins SYT-SSX, binds to both hBRM and BRG1. We have also found that in vivo the C-terminus transactivation QPGY region of SYT can interact with itself. This results in an amplified interaction with hBRM and highlights a possible regulatory function of this domain in cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • COS Cells
  • Chromatin / genetics*
  • Chromatin / metabolism
  • Conserved Sequence*
  • Humans
  • Neurotensin / genetics*
  • Neurotensin / metabolism
  • Protein Structure, Tertiary
  • Proteins / genetics*
  • Proteins / metabolism
  • Proto-Oncogene Proteins
  • Repressor Proteins
  • Transcription Factors / metabolism
  • Vasoactive Intestinal Peptide / metabolism


  • Chromatin
  • Proteins
  • Proto-Oncogene Proteins
  • Repressor Proteins
  • SMARCA2 protein, human
  • SS18 protein, human
  • Transcription Factors
  • stearyl-Nle(17)-neurotensin(6-11)VIP(7-28)
  • Vasoactive Intestinal Peptide
  • Neurotensin