RAD21 and KIAA0196 at 8q24 are amplified and overexpressed in prostate cancer

Genes Chromosomes Cancer. 2004 Jan;39(1):1-10. doi: 10.1002/gcc.10289.


To detect genes that are overexpressed in prostate cancer, a subtracted cDNA library was first constructed from the PC-3 cell line and subsequently screened by using cDNA microarray hybridization. Sixty-eight genes were found to be overexpressed (ratio>3) in PC-3. Half of these genes were in chromosomal regions, which, using comparative genomic hybridization, we previously showed to be gained in PC-3. Subsequently, the expression and copy number of selected genes were studied by quantitative RT-PCR and fluorescence in situ hybridization in prostate cancer cell lines, xenografts, and clinical tumor specimens of benign prostate hyperplasia and untreated as well as hormone-refractory prostate carcinomas. Two genes from chromosomal region 8q24-RAD21 and KIAA0196-showed increased expression in clinical prostate carcinomas and were also amplified in 30-40% of xenografts and hormone-refractory tumors. In addition, the expression of KIAA0196 was significantly (P=0.0051) higher in tumors with the gene amplification than in those without it. The data suggest that KIAA0196 and possibly RAD21 are putative target genes for the common amplification of 8q23-24 in prostate cancer.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Cell Cycle Proteins
  • Cell Line, Tumor
  • Chromosomes, Human, Pair 8 / genetics*
  • DNA, Complementary / genetics
  • DNA-Binding Proteins
  • Gene Amplification*
  • Gene Dosage
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic / genetics*
  • Genes, Neoplasm / genetics*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Male
  • Nuclear Proteins / genetics*
  • Nucleic Acid Hybridization
  • Oligonucleotide Array Sequence Analysis
  • Phosphoproteins / genetics*
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / metabolism
  • Prostatic Neoplasms / pathology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transplantation, Heterologous


  • Cell Cycle Proteins
  • DNA, Complementary
  • DNA-Binding Proteins
  • Nuclear Proteins
  • Phosphoproteins
  • RAD21 protein, human