A protein interaction map of Drosophila melanogaster

Science. 2003 Dec 5;302(5651):1727-36. doi: 10.1126/science.1090289. Epub 2003 Nov 6.


Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence was developed to refine this draft map to a higher confidence map of 4679 proteins and 4780 interactions. Statistical modeling of the network showed two levels of organization: a short-range organization, presumably corresponding to multiprotein complexes, and a more global organization, presumably corresponding to intercomplex connections. The network recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components. This map serves as a starting point for a systems biology modeling of multicellular organisms, including humans.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calcium / metabolism
  • Cell Cycle
  • Cell Differentiation
  • Cloning, Molecular
  • Computational Biology
  • DNA, Complementary
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / metabolism*
  • Drosophila melanogaster / physiology
  • ErbB Receptors / metabolism
  • Genes, Insect
  • Immunity, Innate
  • Mathematics
  • Models, Statistical
  • Photoreceptor Cells, Invertebrate / cytology
  • Protein Binding
  • Protein Interaction Mapping*
  • Proteome*
  • RNA Splicing
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Signal Transduction
  • Transcription, Genetic
  • Two-Hybrid System Techniques


  • DNA, Complementary
  • Drosophila Proteins
  • Proteome
  • RNA, Messenger
  • ErbB Receptors
  • Calcium