Adults and immatures of Ixodes pacificus Cooley & Kohls were collected by flagging vegetation and from lizards during a 3-mo period in the Hualapai Mountain Park, Mohave County, AZ, in 1991. Collections were made at altitudes > or = 2,134 m. Two of 48 gut-salivary gland extracts of adult ticks were positive by IFA using a monoclonal antibody (H5332) specific to Borrelia burgdorferi. These are the first records of I. pacificus and of spirochetes tentatively identified as B. burgdorferi in Arizona.