Abstract
The chloroform extract of the aerial part of Limnophila geoffrayi showed antimycobacterial and antioxidant activities. Bioassay-guided fractionation has led to the isolation of the flavones nevadensin (5,7-dihydroxy-6,8,4'-trimethoxyflavone, 1) and isothymusin (6,7-dimethoxy-5,8,4'-trihydroxyflavone, 2). Both compounds 1 and 2 exhibited inhibition activity against Mycobacterium tuberculosis, with equal MIC value of 200 microg/mL. Only compound 2 exhibited antioxidant activity against the radical scavenging ability of DPPH, with the IC50 value of 7.7 microg/mL. The crude hexane, chloroform and methanol extracts as well as the pure compounds 1 and 2 did not exhibit mutagenic activity in the Bacillus subtilis recassay.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / pharmacology*
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Antioxidants / chemistry*
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Antioxidants / pharmacology*
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Bacillus subtilis / drug effects
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Bacillus subtilis / genetics
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Bacillus subtilis / physiology
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Chloroform / chemistry
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Flavones*
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Flavonoids / chemistry*
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Flavonoids / isolation & purification
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Flavonoids / pharmacology*
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Microbial Sensitivity Tests / methods
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Molecular Structure
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Mutagenicity Tests
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Mycobacterium tuberculosis / drug effects
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Plant Extracts / chemistry
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Plant Leaves / chemistry
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Plant Stems / chemistry
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Scrophulariaceae / chemistry*
Substances
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Anti-Bacterial Agents
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Antioxidants
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Flavones
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Flavonoids
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Plant Extracts
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nevadensin
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Chloroform
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isothymusin