Successful elimination of memory-type CD8+ T cell subsets by the administration of anti-Gr-1 monoclonal antibody in vivo

Cell Immunol. 2003 Aug;224(2):98-105. doi: 10.1016/j.cellimm.2003.08.009.


During investigating the expression of Gr-1 antigen on various subsets of mouse spleen cells, we found that Gr-1 was expressed on memory-type CD8(+)CD44(high)CD62L(high) T cells in addition to granulocytes. Intraperitoneal administration of anti-Gr-1 mAb caused almost complete elimination of Ly-6C(+) memory-type CD8(+) T cells as well as Ly-6G(+) granulocytes. Anti-Gr-1 mAb-treated mouse spleen cells exhibited greatly reduced IFN-gamma production in response to anti-CD3 mAb both in vitro and in vivo. This reduced cytokine production appeared to be derived from elimination of IFN-gamma-producing Gr-1(+)CD8(+) T cells. Indeed, CD8(+) T cells with IFN-gamma-producing activity and cytotoxicity were generated from isolated Gr-1(+)CD8(+) cells but not from Gr-1(-)CD8(+) T cells. We also demonstrated that therapeutic effect of MBL-2 tumor-immunized spleen cells was greatly reduced by anti-Gr-1 mAb-treatment. Thus, we initially demonstrated that anti-Gr-1 mAb might become a good tool to investigate a precise role for memory-type CD8(+) T cells in vivo.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antigens, Differentiation, Myelomonocytic / analysis
  • Antigens, Differentiation, Myelomonocytic / physiology*
  • Antigens, Ly / analysis
  • CD3 Complex / immunology
  • CD8-Positive T-Lymphocytes / immunology*
  • Female
  • Immunization
  • Immunologic Memory*
  • Immunotherapy, Adoptive
  • Interferon-gamma / biosynthesis
  • Lymphocyte Depletion
  • Lymphoma / immunology
  • Mice
  • Mice, Inbred C57BL
  • Neutrophils / physiology


  • Antibodies, Monoclonal
  • Antigens, Differentiation, Myelomonocytic
  • Antigens, Ly
  • CD3 Complex
  • Interferon-gamma