Aurora-B phosphorylation in vitro identifies a residue of survivin that is essential for its localization and binding to inner centromere protein (INCENP) in vivo

J Biol Chem. 2004 Feb 13;279(7):5655-60. doi: 10.1074/jbc.M311299200. Epub 2003 Nov 10.

Abstract

The chromosomal passengers, aurora-B kinase, inner centromere protein (INCENP), and survivin, are essential proteins that have been implicated in the regulation of metaphase chromosome alignment, spindle checkpoint function, and cytokinesis. All three share a common pattern of localization, and it was recently demonstrated that aurora-B, INCENP, and survivin are present in a complex in Xenopus eggs and Saccharomyces cerevisiae. The presence of aurora-B kinase in the complex and its ability to bind the other components directly suggest that INCENP and survivin could potentially be aurora-B substrates. This hypothesis was recently proven for INCENP in vitro. Here we report that human survivin is specifically phosphorylated in vitro by aurora-B kinase at threonine 117 in its carboxyl alpha-helical coil. Mutation of threonine 117 to alanine prevents survivin phosphorylation by aurora-B in vitro but does not alter its localization in HeLa cells. By contrast, a phospho-mimic, in which threonine 117 was mutated to glutamic acid, was unable to localize correctly at any stage in mitosis. Mutation at threonine 117 also prevented immunoprecipitation of INCENP with survivin in vivo. These data suggest that phosphorylation of survivin at threonine 117 by aurora-B may regulate targeting of survivin, and possibly the entire passenger complex, in mammals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Aurora Kinase B
  • Aurora Kinases
  • Cells, Cultured
  • Chromosomal Proteins, Non-Histone / chemistry*
  • Chromosomal Proteins, Non-Histone / metabolism
  • Glutamic Acid / chemistry
  • Glutathione Transferase / metabolism
  • HeLa Cells
  • Humans
  • In Vitro Techniques
  • Inhibitor of Apoptosis Proteins
  • Mass Spectrometry
  • Metaphase
  • Microtubule-Associated Proteins / chemistry*
  • Molecular Sequence Data
  • Mutation
  • Neoplasm Proteins
  • Peptides / chemistry
  • Phosphorylation
  • Precipitin Tests
  • Protein Structure, Tertiary
  • Protein-Serine-Threonine Kinases / physiology*
  • Recombinant Fusion Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Survivin
  • Threonine / chemistry
  • Transfection

Substances

  • BIRC5 protein, human
  • Chromosomal Proteins, Non-Histone
  • INCENP protein, human
  • Inhibitor of Apoptosis Proteins
  • Microtubule-Associated Proteins
  • Neoplasm Proteins
  • Peptides
  • Recombinant Fusion Proteins
  • Survivin
  • Threonine
  • Glutamic Acid
  • Glutathione Transferase
  • AURKB protein, human
  • Aurora Kinase B
  • Aurora Kinases
  • Protein-Serine-Threonine Kinases