Morphological analysis of leucocyte transmigration in the pleural cavity

J Anat. 2003 Oct;203(4):391-404. doi: 10.1046/j.1469-7580.2003.00231.x.


The role that pleural mesothelial cells play in leucocyte transmigration into the pleural cavity was investigated in lipopolysaccharide-stimulated mice. Changes in mesothelial cell morphology and changes in expression of adhesion molecules on mesothelial cells and leucocytes were analysed by light microscopy, immunohistochemistry, transmission electron microscopy (TEM) and immuno-scanning electron microscopy (immuno-SEM). After stimulation, the mesothelial cells separated completely from one another before leucocyte penetration across the mesothelial layer occurred. These changes occurred primarily in the immediate vicinity of ribs, where a large number of leucocytes accumulated. Immuno-SEM showed that the expression of intercellular adhesion molecule-1 (ICAM-1) on the parietal pleural mesothelial cells was significantly up-regulated by lipopolysaccharide stimulation, and that of vascular cell adhesion molecule-1 (VCAM-1) was induced. Both were restricted to the microvilli of the mesothelial cells. By contrast, expression of intercellular adhesion molecule-2 (ICAM-2), platelet/endothelial cell adhesion molecule-1 (PECAM-1), mucosal addressin cell adhesion molecule-1 (MAdCAM-1), endothelial leucocyte adhesion molecule-1 (ELAM-1), peripheral node addressin (PNAd) and fibronectin were not detected. Lymphocyte function associated antigen-1 (LFA-1), macrophage-1 molecule (Mac-1) and very late appearing antigen-4 (VLA-4), all ligands of ICAM-1 and VCAM-1, were present on the transmigrated neutrophils and macrophages. These findings demonstrate that the immediate vicinity of ribs is a source of leucocyte migration into the pleural space.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Surface / analysis
  • Cell Adhesion Molecules
  • Cell Movement / physiology
  • E-Selectin / analysis
  • Epithelial Cells / chemistry
  • Epithelial Cells / physiology*
  • Fibronectins / analysis
  • Immunoglobulins / analysis
  • Immunohistochemistry / methods
  • Integrin alpha4beta1 / analysis
  • Intercellular Adhesion Molecule-1 / analysis
  • Leukocytes / chemistry
  • Leukocytes / physiology*
  • Lipopolysaccharides
  • Lymphocyte Function-Associated Antigen-1 / analysis
  • Macrophage-1 Antigen / analysis
  • Male
  • Membrane Proteins
  • Mice
  • Mice, Inbred ICR
  • Microscopy, Immunoelectron
  • Mucoproteins / analysis
  • Platelet Endothelial Cell Adhesion Molecule-1 / analysis
  • Pleura / immunology*
  • Pleura / ultrastructure
  • Specific Pathogen-Free Organisms
  • Vascular Cell Adhesion Molecule-1 / analysis


  • Antigens, Surface
  • Cell Adhesion Molecules
  • E-Selectin
  • Fibronectins
  • Immunoglobulins
  • Integrin alpha4beta1
  • L-selectin counter-receptors
  • Lipopolysaccharides
  • Lymphocyte Function-Associated Antigen-1
  • Macrophage-1 Antigen
  • Madcam1 protein, mouse
  • Membrane Proteins
  • Mucoproteins
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Vascular Cell Adhesion Molecule-1
  • Intercellular Adhesion Molecule-1