Monitoring genome-wide changes in gene expression in response to endogenous cytokinin reveals targets in Arabidopsis thaliana

FEBS Lett. 2003 Nov 20;554(3):373-80. doi: 10.1016/s0014-5793(03)01194-3.


Cytokinins have been implicated in developmental and growth processes in plants including cell division, chloroplast biogenesis, shoot meristem initiation and senescence. The regulation of these processes requires changes in cytokinin-responsive gene expression. Here, we induced the expression of a bacterial isopentenyl transferase gene, IPT, in transgenic Arabidopsis thaliana seedlings to study the regulation of genome-wide gene expression in response to endogenous cytokinin. Using MPSS (massively parallel signature sequencing) we identified 823 and 917 genes that were up- and downregulated, respectively, following 24 h of IPT induction. When comparing the response to cytokinin after 6 and 24 h, we identified different clusters of genes showing a similar course of regulation. Our study provides researchers with the opportunity to rapidly assess whether genes of interest are regulated by cytokinins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alkyl and Aryl Transferases / genetics
  • Alkyl and Aryl Transferases / metabolism
  • Arabidopsis / genetics*
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / biosynthesis
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism
  • Cytokinins / metabolism
  • Cytokinins / pharmacology*
  • Enzyme Induction / physiology
  • Gene Expression Profiling / methods
  • Gene Expression Regulation, Plant*
  • Genome, Plant*
  • Multigene Family / physiology
  • Plant Roots / genetics
  • Plant Roots / metabolism
  • Plants, Genetically Modified / genetics
  • Plants, Genetically Modified / metabolism
  • RNA, Plant / isolation & purification
  • Signal Transduction
  • Transcription Factors / genetics
  • Transcription Factors / metabolism


  • Arabidopsis Proteins
  • Cytokinins
  • RNA, Plant
  • Transcription Factors
  • Alkyl and Aryl Transferases
  • adenylate isopentenyltransferase