Acute effects of PYY3-36 on food intake and hypothalamic neuropeptide expression in the mouse

Biochem Biophys Res Commun. 2003 Nov 28;311(4):915-9. doi: 10.1016/j.bbrc.2003.10.089.


It has recently been suggested that gut-derived PYY(3-36) may be involved in the central mediation of post-prandial satiety signals. We have examined the acute effects of peripherally administered PYY(3-36) on food intake and hypothalamic gene expression of neuropeptides in mice. A single intraperitoneal injection of PYY(3-36) to mice that had been fasted for 24h resulted in a highly significant reduction in food intake at 6 and 24h post-injection but not at 48h. However, in freely fed mice, food intake was unaltered by PYY(3-36) administration. In the arcuate nucleus POMC mRNA expression was significantly elevated at 6h and remained elevated at 24h following PYY(3-36) injection. By contrast NPY mRNA expression in the arcuate nucleus was suppressed at 6h but not at 24h post-injection. In the lateral hypothalamus there were no differences in MCH mRNA expression at either time point. In conclusion, peripherally administered PYY(3-36) has a suppressive effect on food intake that is more prominent in recently fasted mice and lasts up to 24 h. This is associated with a short-lived suppression of NPY mRNA, a longer lasting increase in POMC mRNA but no change in MCH mRNA expression.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Physiological / drug effects
  • Adaptation, Physiological / physiology
  • Animals
  • Appetite Regulation / drug effects*
  • Appetite Regulation / physiology*
  • Dose-Response Relationship, Drug
  • Gene Expression Regulation / drug effects
  • Hypothalamic Hormones / metabolism
  • Hypothalamus / cytology
  • Hypothalamus / drug effects*
  • Hypothalamus / metabolism*
  • Injections, Intraperitoneal
  • Melanins / metabolism
  • Mice
  • Neuropeptide Y / metabolism
  • Neuropeptides / metabolism*
  • Peptide Fragments / administration & dosage*
  • Peptide YY / administration & dosage*
  • Pituitary Hormones / metabolism
  • Pro-Opiomelanocortin / metabolism


  • Hypothalamic Hormones
  • Melanins
  • Neuropeptide Y
  • Neuropeptides
  • Peptide Fragments
  • Pituitary Hormones
  • peptide YY (13-36)
  • Peptide YY
  • Pro-Opiomelanocortin
  • melanin-concentrating hormone