Adipose tissue growth and regression are regulated by angiopoietin-1

Biochem Biophys Res Commun. 2003 Nov 21;311(3):563-71. doi: 10.1016/j.bbrc.2003.10.007.

Abstract

Adipose tissue is unique in its plasticity, capacity for vascular remodeling, and susceptibility to angiogenesis inhibitors. We hypothesize that these characteristics are enabled by maintaining relatively immature adipose vessels to facilitate vascular/tissue remodeling. We examined the vascular maturation regulators, angiopoietin-1, angiopoietin-2, and tie2 receptor, under different weight-modifying conditions. Adipocytes expressed angiopoietin-1, while adipose endothelial cells expressed angiopoietin-2 and tie2. Adipose tissue growth/regression were associated with decreased angiopoietin-1 mRNA and protein, and tie2 phosphorylation. Angiopoietin-2 and tie2 mRNA levels were stable. Angiopoietin-1 mRNA levels inversely correlated with the rates of change in body weight, independent of the direction (weight gain, loss) or etiology (TNP-470, leptin, and diet restriction) of the weight shift. Obese mice injected with ang1/pcDNA had reduced rates of weight gain and fat pad weights, regardless of the route of plasmid administration (subcutaneous, intramuscular, and intravenous). Thus, angiopoietin-1 may regulate adipose tissue growth, suggesting that vascular maturation alters tissue plasticity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3-L1 Cells
  • Adipose Tissue / physiology*
  • Angiopoietin-1 / physiology*
  • Angiopoietin-2 / biosynthesis
  • Animals
  • Blotting, Northern
  • Blotting, Western
  • Cyclohexanes
  • Leptin / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Obese
  • O-(Chloroacetylcarbamoyl)fumagillol
  • Plasmids / metabolism
  • Precipitin Tests
  • RNA, Messenger / metabolism
  • Receptor, TIE-2 / biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sesquiterpenes / pharmacology
  • Time Factors
  • Tissue Distribution
  • Transgenes

Substances

  • Angiopoietin-1
  • Angiopoietin-2
  • Cyclohexanes
  • Leptin
  • RNA, Messenger
  • Sesquiterpenes
  • Receptor, TIE-2
  • O-(Chloroacetylcarbamoyl)fumagillol