Determination of hamamelitannin, catechins and gallic acid in witch hazel bark, twig and leaf by HPLC

J Pharm Biomed Anal. 2003 Nov 24;33(4):539-44. doi: 10.1016/s0731-7085(03)00303-0.

Abstract

An HPLC method for the determination of hamamelitannin, catechins and gallic acid in witch hazel bark, twig and leaf has been developed. The separation system consisted of a C18 reversed-phase column, a gradient elution system of methanol/water and orthophosphoric acid, and a photodiode array detector. The concentrations of hamamelitannin, gallic acid, (+)-gallocatechin and (+)-catechin were 4.77, 0.59, 0.22 and 0.39% (w/w), respectively, in the bark. Hamamelitannin and catechins were also detected in the leaves at concentration less than 0.04% (w/w). This method is simple, sensitive and reproducible, ideally suited for rapid, routine analysis.

MeSH terms

  • Catechin / analysis*
  • Chromatography, High Pressure Liquid / methods
  • Gallic Acid / analogs & derivatives*
  • Gallic Acid / analysis*
  • Hamamelis*
  • Hexoses / analysis*
  • Plant Bark
  • Plant Extracts / analysis
  • Plant Leaves
  • Plant Stems

Substances

  • Hexoses
  • Plant Extracts
  • Gallic Acid
  • Catechin
  • hamamelitannin