Synthesis of deuterated isotopomers of 7alpha- and (25R,S)-26-hydroxycholesterol, internal standards for in vivo determination of the two biosynthetic pathways of bile acids

Steroids. 2003 Oct;68(9):733-8. doi: 10.1016/s0039-128x(03)00116-8.

Abstract

Deuterated isotopomers of 7alpha- and (25R,S)-26-hydroxycholesterol, internal standards for in vivo determination of the two biosynthetic pathways of bile acids formation from cholesterol, were prepared from [2,2,3,4,4,6-2H(6)]-cholesterol and (20S)-[7,7,21,21-2H(4)]-3beta-(tert-butyldimethylsilyl)oxy-20-methylpregna-5-en-21-ol, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bile Acids and Salts / metabolism*
  • Deuterium / chemistry*
  • Hydroxycholesterols / chemistry*
  • Isotope Labeling*
  • Magnetic Resonance Spectroscopy
  • Molecular Structure
  • Stereoisomerism

Substances

  • Bile Acids and Salts
  • Hydroxycholesterols
  • cholest-5-ene-3 beta,26-diol
  • cholest-5-en-3 beta,7 alpha-diol
  • Deuterium