Immobilized culture of nonadherent cells on an oleyl poly(ethylene glycol) ether-modified surface

Biotechniques. 2003 Nov;35(5):1014-8, 1020-1. doi: 10.2144/03355rr01.


Microarrays of living cells are an emerging tool in systems such as reverse transfection. These studies are limited to adherent cells partly because of the difficulty of cell immobilization. Using a newly developed reagent, the biocompatible anchor for membrane (BAM), we show herein the rapid and strong attachment of living nonadherent cells and adherent cells on BAM-modified surfaces. Normal cellular growth was observed for over 7 days on BAM-modified surfaces. We expect this methodology to greatly expand the scope of current cell microarray technology.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Animals
  • Biocompatible Materials / chemical synthesis*
  • Biocompatible Materials / chemistry
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods
  • Cell Adhesion / physiology*
  • Cell Culture Techniques / methods*
  • Cell Division / physiology*
  • Cell Survival / physiology
  • Cells, Immobilized / physiology*
  • Ether / chemistry*
  • Humans
  • Jurkat Cells
  • K562 Cells
  • Membranes, Artificial
  • Mice
  • NIH 3T3 Cells
  • Polyethylene Glycols / chemistry*
  • Surface Properties


  • Biocompatible Materials
  • Membranes, Artificial
  • Ether
  • Polyethylene Glycols