Immobilized culture of nonadherent cells on an oleyl poly(ethylene glycol) ether-modified surface

Biotechniques. 2003 Nov;35(5):1014-8, 1020-1. doi: 10.2144/03355rr01.

Abstract

Microarrays of living cells are an emerging tool in systems such as reverse transfection. These studies are limited to adherent cells partly because of the difficulty of cell immobilization. Using a newly developed reagent, the biocompatible anchor for membrane (BAM), we show herein the rapid and strong attachment of living nonadherent cells and adherent cells on BAM-modified surfaces. Normal cellular growth was observed for over 7 days on BAM-modified surfaces. We expect this methodology to greatly expand the scope of current cell microarray technology.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Animals
  • Biocompatible Materials / chemical synthesis*
  • Biocompatible Materials / chemistry
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods
  • Cell Adhesion / physiology*
  • Cell Culture Techniques / methods*
  • Cell Division / physiology*
  • Cell Survival / physiology
  • Cells, Immobilized / physiology*
  • Ether / chemistry*
  • Humans
  • Jurkat Cells
  • K562 Cells
  • Membranes, Artificial
  • Mice
  • NIH 3T3 Cells
  • Polyethylene Glycols / chemistry*
  • Surface Properties

Substances

  • Biocompatible Materials
  • Membranes, Artificial
  • Ether
  • Polyethylene Glycols