A generational study of glyphosate-tolerant soybeans on mouse fetal, postnatal, pubertal and adult testicular development

Food Chem Toxicol. 2004 Jan;42(1):29-36. doi: 10.1016/j.fct.2003.08.003.


The health safety of transgenic soybeans (glyphosate-tolerant or Roundup Ready) was studied using the mammalian testis (mouse model) as a sensitive biomonitor of potential toxic effects. Pregnant mice were fed a transgenic soybean or a non-transgenic (conventional) diet through gestation and lactation. After weaning, the young male mice were maintained on the respective diets. At 8, 16, 26, 32, 63 and 87 days after birth, three male mice and an adult reference mouse were killed, the testes surgically removed, and the cell populations measured by flow cytometry. Multi-generational studies were conducted in the same manner. The results showed that the transgenic foodstuffs had no effect on macromolecular synthesis or cell growth and differentiation as evidenced by no differences in the percentages of testicular cell populations (haploid, diploid, and tetraploid) between the transgenic soybean-fed mice and those fed the conventional diet. Additionally, there were no differences in litter sizes and body weights of the two groups. It was concluded that the transgenic soybean diet had no negative effect on fetal, postnatal, pubertal or adult testicular development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Body Weight / drug effects
  • Cell Wall / metabolism
  • Diet
  • Drug Resistance
  • Female
  • Flow Cytometry
  • Glycine / analogs & derivatives*
  • Glycine / toxicity*
  • Herbicides / toxicity*
  • Kinetics
  • Male
  • Mice
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Plants, Genetically Modified / genetics*
  • Plants, Genetically Modified / toxicity*
  • Pregnancy
  • Sexual Maturation / drug effects*
  • Soybeans / chemistry
  • Soybeans / genetics*
  • Soybeans / toxicity*
  • Testis / drug effects
  • Testis / embryology
  • Testis / growth & development*


  • Herbicides
  • glyphosate
  • Glycine