Retraction in amoeboid cell motility powered by cytoskeletal dynamics

Science. 2003 Nov 21;302(5649):1405-7. doi: 10.1126/science.1089129.

Abstract

Cells crawl by coupling protrusion of their leading edge with retraction of their cell body. Protrusion is generated by the polymerization and bundling of filaments, but the mechanism of retraction is less clear. We have reconstituted retraction in vitro by adding Yersinia tyrosine phosphatase to the major sperm protein-based motility apparatus assembled from Ascaris sperm extracts. Retraction in vitro parallels that observed in vivo and is generated primarily by disassembly and rearrangement of the cytoskeleton. Therefore, cytoskeletal dynamics alone, unassisted by conventional motors, are able to generate both of these central components of amoeboid locomotion.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / physiology
  • Adenosine Triphosphate / metabolism
  • Adenosine Triphosphate / pharmacology
  • Animals
  • Ascaris suum / cytology*
  • Ascaris suum / physiology
  • Biopolymers
  • Cell Adhesion
  • Cell Extracts
  • Cell Movement / physiology*
  • Cytoplasmic Vesicles / physiology
  • Cytoskeleton / physiology*
  • Helminth Proteins / chemistry
  • Helminth Proteins / metabolism
  • Helminth Proteins / physiology*
  • Hydrogen-Ion Concentration
  • Male
  • Myosins / physiology
  • Phosphorylation
  • Protein Tyrosine Phosphatases / metabolism
  • Pseudopodia / physiology
  • Spermatozoa / physiology
  • Spermatozoa / ultrastructure
  • Yersinia enterocolitica / enzymology

Substances

  • Actins
  • Biopolymers
  • Cell Extracts
  • Helminth Proteins
  • major sperm protein, nematode
  • Adenosine Triphosphate
  • Protein Tyrosine Phosphatases
  • Myosins