Synthesis of L-ascorbic acid in the phloem

BMC Plant Biol. 2003 Nov 24;3:7. doi: 10.1186/1471-2229-3-7.

Abstract

Background: Although plants are the main source of vitamin C in the human diet, we still have a limited understanding of how plants synthesise L-ascorbic acid (AsA) and what regulates its concentration in different plant tissues. In particular, the enormous variability in the vitamin C content of storage organs from different plants remains unexplained. Possible sources of AsA in plant storage organs include in situ synthesis and long-distance transport of AsA synthesised in other tissues via the phloem. In this paper we examine a third possibility, that of synthesis within the phloem.

Results: We provide evidence for the presence of AsA in the phloem sap of a wide range of crop species using aphid stylectomy and histochemical approaches. The activity of almost all the enzymes of the primary AsA biosynthetic pathway were detected in phloem-rich vascular exudates from Cucurbita pepo fruits and AsA biosynthesis was demonstrated in isolated phloem strands from Apium graveolens petioles incubated with a range of precursors (D-glucose, D-mannose, L-galactose and L-galactono-1,4-lactone). Phloem uptake of D-[U-14C]mannose and L-[1-14C]galactose (intermediates of the AsA biosynthetic pathway) as well as L-[1-14C]AsA and L-[1-14C]DHA, was observed in Nicotiana benthamiana leaf discs.

Conclusions: We present the novel finding that active AsA biosynthesis occurs in the phloem. This process must now be considered in the context of mechanisms implicated in whole plant AsA distribution. This work should provoke studies aimed at elucidation of the in vivo substrates for phloem AsA biosynthesis and its contribution to AsA accumulation in plant storage organs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apium / chemistry
  • Apium / enzymology
  • Apium / metabolism
  • Ascorbic Acid / biosynthesis*
  • Autoradiography
  • Carbohydrate Metabolism
  • Carbon Radioisotopes
  • Chromatography, High Pressure Liquid
  • Cucurbitaceae / chemistry
  • Cucurbitaceae / enzymology
  • Cucurbitaceae / metabolism
  • Galactose / metabolism
  • Galactose Dehydrogenases / metabolism
  • Glucose / metabolism
  • Glucose-6-Phosphate Isomerase / metabolism
  • Hexokinase / metabolism
  • Mannose / metabolism
  • Mannose-6-Phosphate Isomerase / metabolism
  • Plant Extracts / chemistry
  • Plant Extracts / metabolism
  • Plant Leaves / metabolism
  • Plant Structures / chemistry
  • Plant Structures / enzymology
  • Plant Structures / metabolism*
  • Pyrophosphatases / metabolism
  • Tobacco / metabolism

Substances

  • Carbon Radioisotopes
  • Plant Extracts
  • Galactose Dehydrogenases
  • galactose dehydrogenase
  • Hexokinase
  • Pyrophosphatases
  • Mannose-6-Phosphate Isomerase
  • Glucose-6-Phosphate Isomerase
  • Glucose
  • Mannose
  • Ascorbic Acid
  • Galactose