Normal development of the gut-associated lymphoid tissue except Peyer's patch in MyD88-deficient mice

Scand J Immunol. 2003 Dec;58(6):620-7. doi: 10.1111/j.1365-3083.2003.01346.x.


MyD88 is a key adaptor molecule for signalling via Toll-like receptors (TLRs) and the response to gut commensal microbes. To investigate the role of TLRs/MyD88 pathway in the development of the gut-associated lymphoid tissue (GALT), we examined the development of Peyer's patches (PPs) and cryptopatch (CP), and also one of effector compartment, intraepithelial lymphocyte (IEL) in MyD88-/-, TLR2-/- and TLR4-/- mice. In MyD88-/- mice, the organogenesis of PPs was not disturbed. However, PPs in 2-week-old MyD88-/- mice were significantly smaller than those in MyD88+/- mice. Also, in 2-week-old TLR4-/-, but not TLR2-/- mice, PPs did not develop rapidly. The development of PPs in MyD88-/- and TLR4-/- mice was completely recovered in 10 weeks. PP cells from MyD88-/- mice showed significant decrease in proliferation when stimulated with lipopolysaccharide. The development of CP and IEL was also normal in 10-week-old MyD88-/- mice. These results suggest that the TLRs/MyD88 pathway might be involved in the development of PPs only at early postnatal stage, and TLRs/MyD88-independent signalling is critically involved in the development of GALT in adult mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Animals
  • Antigens, Differentiation / physiology*
  • Cell Division / drug effects
  • Intestinal Mucosa / immunology*
  • Lipopolysaccharides / pharmacology
  • Lymphoid Tissue / growth & development*
  • Membrane Glycoproteins / physiology
  • Mice
  • Mice, Inbred C57BL
  • Myeloid Differentiation Factor 88
  • Peyer's Patches / growth & development*
  • Receptors, Cell Surface / physiology
  • Receptors, Immunologic / physiology*
  • Spleen / growth & development
  • Toll-Like Receptor 2
  • Toll-Like Receptor 4
  • Toll-Like Receptors


  • Adaptor Proteins, Signal Transducing
  • Antigens, Differentiation
  • Lipopolysaccharides
  • Membrane Glycoproteins
  • Myd88 protein, mouse
  • Myeloid Differentiation Factor 88
  • Receptors, Cell Surface
  • Receptors, Immunologic
  • Toll-Like Receptor 2
  • Toll-Like Receptor 4
  • Toll-Like Receptors