Evaluation of the analytic performances of the new HPLC system HLC-723 G7 for the measurement of hemoglobin A1c

Clin Biochem. 2003 Nov;36(8):607-10. doi: 10.1016/s0009-9120(03)00116-4.

Abstract

Objectives: The analytical performance of a new automated HPLC system, for the determination of HbA1C in blood (Tosoh HLC-723 G7), was studied.

Design and methods: The study design included the evaluation of imprecision, linearity, interference and carryover. Comparison study was performed by comparing HbA1C results with those obtained from an established method (Bio-Rad Variant II).

Results: Total imprecision was less than 1.34% and the results were linear up to 17.2% HbA1C. The method showed a wide analytical range, and no carryover between specimens. Comparison study yielded, r=0.989, Sy.x=0.255, regression equation (y=0.9895x-0.35); Bland-Altman plot showed a mean bias=- 0.43% HbA1C with confidence limits ranging from -0.48% to -0.38% HbA1C. The presence of abnormal hemoglobin was clearly revealed, and no interference from labile HbA1C was apparent.

Conclusion: The HLC-723 G7 instrument seems to be a reliable system for routine assay of HbA1C.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Bilirubin / blood
  • Chromatography, High Pressure Liquid / instrumentation*
  • Chromatography, High Pressure Liquid / methods*
  • Chromatography, High Pressure Liquid / statistics & numerical data
  • Glycated Hemoglobin A / analysis*
  • Hemoglobins, Abnormal / analysis
  • Humans
  • Triglycerides / blood

Substances

  • Glycated Hemoglobin A
  • Hemoglobins, Abnormal
  • Triglycerides
  • Bilirubin