HPLC analysis of some sulphur compounds in saliva: comparison between healthy subjects and periodontopathic patients

Clin Chim Acta. 2003 Dec;338(1-2):57-60. doi: 10.1016/j.cccn.2003.07.019.


Background: Saliva is an easily available biological material that is not commonly analysed in clinical chemistry, while it could give useful information especially in several oral diseases.

Methods: In this work, the sulphur containing compounds cysteine, cysteinylglycine and glutathione were analysed in saliva of control subjects and periodontopathic subjects by a HPLC method. The detection limit of the method is 0.5, 0.1 and 0.1 micromol/l for cysteine, cysteinylglycine and glutathione, respectively, and it is linear up to 10 mmol/l.

Results: the median values for the control group are 1.2 micromol/l for cysteine and glutathione and 0.4 micromol/l for cysteinylglycine while those of periodontopathic patients are significantly increased (4.4, 2.1 and 11.0 micromol/l for cysteine, cysteinylglycine and glutathione, respectively).

MeSH terms

  • Adult
  • Aged
  • Chromatography, High Pressure Liquid
  • Cysteine / analysis
  • Cysteine / metabolism
  • Dipeptides / analysis
  • Dipeptides / metabolism
  • Female
  • Glutathione / analysis
  • Glutathione / metabolism
  • Humans
  • Male
  • Middle Aged
  • Periodontal Diseases / metabolism*
  • Saliva / chemistry*
  • Saliva / metabolism
  • Sulfur Compounds / analysis*
  • Sulfur Compounds / metabolism


  • Dipeptides
  • Sulfur Compounds
  • cysteinylglycine
  • Glutathione
  • Cysteine