Phytanic acid alpha-oxidation in human cultured skin fibroblasts

Biochim Biophys Acta. 1992 Dec 10;1180(2):221-4. doi: 10.1016/0925-4439(92)90072-u.

Abstract

We studied the oxidation of [1-14C]phytanic acid, 3-methyl substituted fatty acid, to pristanic acid and 14CO2 in human skin fibroblasts. The specific activity for alpha-oxidation of phytanic acid in peroxisomes was 29- and 124-fold higher than mitochondria and endoplasmic reticulum. This finding demonstrates for the first time the presence of fatty acid alpha-oxidation enzyme system in peroxisomes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Fractionation
  • Cells, Cultured
  • Coenzyme A Ligases / metabolism
  • Epoxy Compounds / pharmacology
  • Fatty Acids / metabolism
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Humans
  • Oxidation-Reduction
  • Phytanic Acid / pharmacology*
  • Repressor Proteins*
  • Saccharomyces cerevisiae Proteins*
  • Skin / drug effects*
  • Skin / enzymology
  • Skin / metabolism
  • Subcellular Fractions / metabolism

Substances

  • Epoxy Compounds
  • Fatty Acids
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Phytanic Acid
  • pristanic acid
  • ethyl 2-(5-(4-chlorophenyl)pentyl)oxiran-2-carboxylate
  • Coenzyme A Ligases
  • lignoceroyl-CoA ligase
  • FAA2 protein, S cerevisiae
  • long-chain-fatty-acid-CoA ligase