The expression of a rab-related gene, rab18, is induced by abscisic acid during the cold acclimation process of Arabidopsis thaliana (L.) Heynh

Plant Mol Biol. 1992 Dec;20(5):951-62. doi: 10.1007/BF00027165.


We have isolated a rab-related (responsive to ABA) gene, rab18 from Arabidopsis thaliana. The gene encodes a hydrophilic, glycine-rich protein (18.5 kDa), which contains the conserved serine- and lysine-rich domains characteristic of similar RAB proteins in other plant species. The rab18 mRNA accumulates in plants exposed to low temperature, water stress or exogenous ABA but not in plants subjected to heat shock. This stress-related accumulation of the rab18 mRNA is markedly decreased in the ABA-synthesis mutant aba-1, the ABA-response mutant abi-1 or in wild-type plants treated with the carotenoid synthesis inhibitor, fluridone. Exogenous ABA treatment can induce the rab18 mRNA in the aba-1 mutant but not in the abi-1 mutant. These results provide direct genetic evidence for the ABA-dependent regulation of the rab18 gene in A. thaliana.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscisic Acid / pharmacology*
  • Acclimatization
  • Amino Acid Sequence
  • Arabidopsis / drug effects
  • Arabidopsis / genetics*
  • Arabidopsis / physiology
  • Arabidopsis Proteins*
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • Cold Temperature
  • DNA / genetics
  • DNA / isolation & purification
  • Gene Expression Regulation / drug effects*
  • Molecular Sequence Data
  • Molecular Weight
  • Oligodeoxyribonucleotides
  • Plant Proteins / genetics*
  • Pyridones / pharmacology
  • RNA, Messenger / metabolism
  • Sequence Homology, Amino Acid
  • Water
  • rab GTP-Binding Proteins*


  • Arabidopsis Proteins
  • Oligodeoxyribonucleotides
  • Plant Proteins
  • Pyridones
  • RNA, Messenger
  • rab18 protein, Arabidopsis
  • Water
  • fluridone
  • Abscisic Acid
  • DNA
  • rab GTP-Binding Proteins

Associated data

  • GENBANK/X68042