New plant binary vectors with selectable markers located proximal to the left T-DNA border

Plant Mol Biol. 1992 Dec;20(6):1195-7. doi: 10.1007/BF00028908.


Five new binary vectors have been constructed which have the following features: (1) different plant selectable markers including neomycin phosphotransferase (nptII), hygromycin phosphotransferase (hpt), dihydrofolate reductase (dhfr), phosphinothricin acetyl transferase (bar), and bleomycin resistance (ble); (2) selectable markers are located near the T-DNA left border and; (3) selectable marker and beta-glucuronidase (uidA) reporter genes are divergently organized for efficient expression, and can easily be removed or replaced as needed.

MeSH terms

  • Agrobacterium tumefaciens / genetics*
  • Arabidopsis / genetics
  • Genetic Markers
  • Genetic Vectors*
  • Selection, Genetic
  • Transformation, Genetic


  • Genetic Markers