Oxidative stress in hypertensive,diabetic, and diabetic hypertensive rats

Am J Hypertens. 2003 Dec;16(12):1049-52. doi: 10.1016/j.amjhyper.2003.07.013.


Background: Reactive oxygen species play a key role in the formation of endothelial dysfunction accompanying diabetes mellitus, hypertension, and other cardiovascular diseases.

Method: This study compares oxidative stress (OS) in Wistar-Kyoto rats (WKY), spontaneously hypertensive rats (SHR), non-insulin-dependent Cohen Diabetic rats (CDR), and Cohen Rosenthal diabetic hypertensive rats (CRDH), a unique animal model of both diabetes and hypertension. The OS was evaluated with a newly developed thermochemiluminiscence (TCL) analyzer (Lumitest Ltd., Nesher, Israel) that measures the oxidizability (ie, susceptibility to oxidation) of a test sample.

Results: The TCL oxidizability test results of sera from the different rats groups showed a time-dependent increase in TCL of up to 145% +/- 7% for WKY, 160% +/- 8% for SHR, 179% +/- 12% for CDR, and 226% +/- 15% for CRDH. These results were significant: P <.001 for SHR and CDR and P <.0001 for CRDH in comparison to WKY. Lipid peroxide levels also increased in each strain of rats: to 80 +/- 7.8 nmol/mL in WKY, 104 +/- 10.1 nmol/mL in SHR, 110 +/- 9.4 nmol/mL in CDR, and 167 +/- 11.7 nmol/mL in CRDH. These results were also significant: P <.001 for SHR, CDR and CRDH in comparison to WKY.

Conclusion: The combination of hypertension and diabetes is accompanied by higher oxidative stress than that seen with either disorder alone.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Diabetes Complications
  • Diabetes Mellitus / physiopathology*
  • Hypertension / complications
  • Hypertension / physiopathology*
  • Lipid Peroxides / metabolism
  • Luminescent Measurements
  • Models, Animal
  • Oxidative Stress / physiology*
  • Rats
  • Rats, Inbred SHR
  • Rats, Inbred WKY
  • Reactive Oxygen Species / metabolism*


  • Lipid Peroxides
  • Reactive Oxygen Species