Single-unit activity of paraventricular nucleus neurons in response to intero- and exteroceptive stressors in conscious, freely moving rats

Abstract

Extracellular recordings of 114 neurons in the hypothalamic paraventricular nucleus (PVN) of conscious, freely moving male rats were performed using a movable electrode system. Single-unit activities were examined for their spontaneous firing patterns and responses to intero- and exteroceptive stressors, including disturbance in arterial blood pressure, water deprivation, air-jet stimulation, and systemic administration of cholecystokinin-8 (CCK). PVN neurons were assigned to one of two groups on the basis of their spontaneous firing patterns: phasic (n=29) and non-phasic (n=85). Intravenous (i.v.) administration of phenylephrine (8 microg/kg) resulted in the inhibition of a greater percentage of phasic-type (88.9%; 24/27) than non-phasic-type neurons (14.9%; 11/74). Most phasic-type neurons showed excitation in response to i.v. administration of sodium nitroprusside (20 microg/kg, 66.7%; 18/27) and water deprivation (15 h, 77.8%; 7/9) when compared to non-phasic-type neurons. Conversely, a greater number of non-phasic-type neurons showed excitation in response to air-jet stimulation (5 l/min, 10 s, 29.0%; 20/69) and to i.v. administration of CCK (5 microg/kg, 24.5%; 11/45) when compared to phasic-type neurons. However, most non-phasic-type neurons that demonstrated excitation in response to i.v. administration of CCK (88.9%; 8/9) did not respond to air-jet stimulation. The present study indicated that phasically firing neurons recorded from the PVN in conscious, freely moving rats are putative vasopressin-secreting neurons on the basis of their responses to intero- and exteroceptive stressors. These data contribute to our understanding of local neural mechanisms within the PVN that are responsible for stress responses in conscious rats.