Characterization of vnr-13, the first alphaherpesvirus gene of the bcl-2 family

Virology. 2003 Nov 25;316(2):256-66. doi: 10.1016/j.virol.2003.08.014.


The Bcl-2 family, including antiapoptotic and proapoptotic members, plays key regulating roles in programmed cell death. We report the characterization of a new member of the bcl-2 family, encoded by herpesvirus of turkeys (HVT). The product of this gene shares 80% homology with Nr-13, an apoptosis inhibitor, which is overexpressed in avian cells transformed by the v-src oncogene. This new gene, that we propose to call vnr-13, is the first member of the bcl-2 family to be isolated among alpha-herpesviruses. Results from cells expressing the HVT-vnr-13 gene product show that the encoded protein inhibits apoptosis and also reduces the rate of cellular proliferation. Contrary to all bcl-2 homologues found in gamma-herpesvirus, which are intronless, vnr-13 has the same organization as the cellular nr-13 gene. Hence, the HVT vnr-13 gene may have been acquired from a reverse transcriptase product of an unspliced precursor RNA, or via direct recombination with the host chromosomal DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alphaherpesvirinae / genetics*
  • Amino Acid Sequence
  • Animals
  • Apoptosis
  • Avian Proteins*
  • Base Sequence
  • COS Cells
  • Cell Cycle
  • Chick Embryo
  • Genes, Viral / physiology*
  • Humans
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics*
  • Membrane Proteins / physiology
  • Molecular Sequence Data
  • Open Reading Frames
  • Turkeys / virology*


  • Avian Proteins
  • Membrane Proteins
  • NR13 protein, avian