Abstract
T cell factor (TCF)-1 is a T-cell-specific transcription factor that is expressed at all stages of T cell development. Deletion of the TCF-1 gene leads to an early block in thymocyte maturation precluding the study of its role at late stages and during positive selection of T cells. In this report we show that beta-catenin, a central effector in the Wnt-TCF-1 signaling pathway, regulates late stages of T cell development. Specifically, transgenic expression of beta-catenin enhances generation of mature thymocytes. Interestingly, CD8-expressing mature thymocytes were affected to a greater extent than CD4-expressing cells. These data suggest that the Wnt-beta-catenin-TCF-1 signaling pathway plays a role during late stages of T cell development.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Animals, Newborn
-
Antibodies, Monoclonal / immunology
-
Antibodies, Monoclonal / pharmacology
-
Antigens, CD / analysis
-
Antigens, CD / metabolism
-
Blotting, Western
-
Bone Marrow Transplantation / immunology
-
CD3 Complex / immunology
-
CD4 Antigens / analysis
-
CD4 Antigens / metabolism
-
CD8 Antigens / analysis
-
CD8 Antigens / metabolism
-
Cell Count
-
Cell Death / genetics
-
Cell Differentiation / immunology*
-
Cell Differentiation / physiology
-
Cell Division / drug effects
-
Cell Division / genetics
-
Cytoskeletal Proteins / genetics
-
Cytoskeletal Proteins / immunology
-
Cytoskeletal Proteins / physiology*
-
Flow Cytometry
-
Gene Expression
-
Heterozygote
-
Histocompatibility Antigens Class I / genetics
-
Histocompatibility Antigens Class I / immunology
-
Histocompatibility Antigens Class II / genetics
-
Histocompatibility Antigens Class II / immunology
-
Homozygote
-
Humans
-
Interleukin-2 / pharmacology
-
Lymphocyte Subsets / chemistry
-
Lymphocyte Subsets / immunology
-
Membrane Glycoproteins / genetics
-
Mice
-
Mice, Inbred C57BL
-
Mice, Knockout
-
Mice, Transgenic
-
Mutation
-
Perforin
-
Phenotype
-
Pore Forming Cytotoxic Proteins
-
Reverse Transcriptase Polymerase Chain Reaction
-
Signal Transduction / immunology
-
Signal Transduction / physiology
-
T-Lymphocyte Subsets / chemistry
-
T-Lymphocyte Subsets / immunology
-
T-Lymphocytes / immunology*
-
T-Lymphocytes / metabolism
-
Thymus Gland / chemistry
-
Thymus Gland / cytology
-
Thymus Gland / metabolism
-
Trans-Activators / genetics
-
Trans-Activators / immunology
-
Trans-Activators / physiology*
-
beta 2-Microglobulin / genetics
-
beta 2-Microglobulin / immunology
-
beta Catenin
Substances
-
Antibodies, Monoclonal
-
Antigens, CD
-
CD3 Complex
-
CD4 Antigens
-
CD8 Antigens
-
CTNNB1 protein, human
-
CTNNB1 protein, mouse
-
Cytoskeletal Proteins
-
Histocompatibility Antigens Class I
-
Histocompatibility Antigens Class II
-
Interleukin-2
-
Membrane Glycoproteins
-
Pore Forming Cytotoxic Proteins
-
Trans-Activators
-
beta 2-Microglobulin
-
beta Catenin
-
Perforin