The Streptomyces coelicolor polynucleotide phosphorylase homologue, and not the putative poly(A) polymerase, can polyadenylate RNA

J Bacteriol. 2003 Dec;185(24):7273-8. doi: 10.1128/JB.185.24.7273-7278.2003.

Abstract

A protein containing a nucleotidyltransferase motif characteristic of poly(A) polymerases has been proposed to polyadenylate RNA in Streptomyces coelicolor (P. Bralley and G. H. Jones, Mol. Microbiol. 40:1155-1164, 2001). We show that this protein lacks poly(A) polymerase activity and is instead a tRNA nucleotidyltransferase that repairs CCA ends of tRNAs. In contrast, a Streptomyces coelicolor polynucleotide phosphorylase homologue that exhibits polyadenylation activity may account for the poly(A) tails found in this organism.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Molecular Sequence Data
  • Polyadenylation
  • Polynucleotide Adenylyltransferase / genetics
  • Polynucleotide Adenylyltransferase / metabolism
  • Polyribonucleotide Nucleotidyltransferase / genetics*
  • Polyribonucleotide Nucleotidyltransferase / metabolism*
  • RNA, Bacterial / metabolism
  • RNA, Transfer / metabolism
  • Streptomyces / enzymology*
  • Streptomyces / genetics*

Substances

  • RNA, Bacterial
  • RNA, Transfer
  • Polynucleotide Adenylyltransferase
  • Polyribonucleotide Nucleotidyltransferase