Abstract
A protein containing a nucleotidyltransferase motif characteristic of poly(A) polymerases has been proposed to polyadenylate RNA in Streptomyces coelicolor (P. Bralley and G. H. Jones, Mol. Microbiol. 40:1155-1164, 2001). We show that this protein lacks poly(A) polymerase activity and is instead a tRNA nucleotidyltransferase that repairs CCA ends of tRNAs. In contrast, a Streptomyces coelicolor polynucleotide phosphorylase homologue that exhibits polyadenylation activity may account for the poly(A) tails found in this organism.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Molecular Sequence Data
-
Polyadenylation
-
Polynucleotide Adenylyltransferase / genetics
-
Polynucleotide Adenylyltransferase / metabolism
-
Polyribonucleotide Nucleotidyltransferase / genetics*
-
Polyribonucleotide Nucleotidyltransferase / metabolism*
-
RNA, Bacterial / metabolism
-
RNA, Transfer / metabolism
-
Streptomyces / enzymology*
-
Streptomyces / genetics*
Substances
-
RNA, Bacterial
-
RNA, Transfer
-
Polynucleotide Adenylyltransferase
-
Polyribonucleotide Nucleotidyltransferase