Colonial differentiation in Streptomyces coelicolor depends on translation of a specific codon within the adpA gene

J Bacteriol. 2003 Dec;185(24):7291-6. doi: 10.1128/JB.185.24.7291-7296.2003.

Abstract

We identified adpA as an araC-like regulatory gene needed for colonial morphogenesis in Streptomyces coelicolor and showed that its activity depended on a unique TTA triplet corresponding to the leucyl-tRNA gene (bldA). These findings partially explained the dependence of aerial mycelium formation on a rare tRNA that is postulated to have developmental control functions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Codon
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Gene Expression Regulation, Bacterial
  • Mycelium / genetics*
  • Phenotype
  • Promoter Regions, Genetic
  • Protein Biosynthesis
  • RNA, Bacterial
  • RNA, Transfer, Leu / genetics
  • Streptomyces / genetics*
  • Streptomyces / growth & development*
  • Streptomyces / metabolism
  • Trans-Activators / genetics*
  • Trans-Activators / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transcription, Genetic

Substances

  • AdpA protein, Streptomyces griseus
  • Bacterial Proteins
  • BldA tRNA, Streptomyces coelicolor
  • Codon
  • DNA-Binding Proteins
  • RNA, Bacterial
  • RNA, Transfer, Leu
  • RamR protein, Streptomyces
  • Trans-Activators
  • Transcription Factors