Novel methyltransferase for modified uridine residues at the wobble position of tRNA

Mol Cell Biol. 2003 Dec;23(24):9283-92. doi: 10.1128/MCB.23.24.9283-9292.2003.

Abstract

We have identified a novel tRNA methyltransferase in Saccharomyces cerevisiae that we designate Trm9. This enzyme, the product of the YML014w gene, catalyzes the esterification of modified uridine nucleotides, resulting in the formation of 5-methylcarbonylmethyluridine in tRNA(Arg3) and 5-methylcarbonylmethyl-2-thiouridine in tRNA(Glu). In intact yeast cells, disruption of the TRM9 gene results in the complete loss of these modified wobble bases and increased sensitivity at 37 degrees C to paromomycin, a translational inhibitor. These results suggest a role for this potentially reversible methyl esterification reaction when cells are under stress.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA, Fungal / genetics
  • Gene Deletion
  • Genes, Fungal
  • Methylation
  • Molecular Sequence Data
  • Mutation
  • Protein Biosynthesis
  • RNA, Fungal / chemistry
  • RNA, Fungal / metabolism*
  • RNA, Transfer / chemistry
  • RNA, Transfer / metabolism*
  • RNA, Transfer, Arg / chemistry
  • RNA, Transfer, Arg / metabolism
  • RNA, Transfer, Glu / chemistry
  • RNA, Transfer, Glu / metabolism
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Sequence Homology, Amino Acid
  • Substrate Specificity
  • Temperature
  • Uridine / chemistry
  • tRNA Methyltransferases / genetics
  • tRNA Methyltransferases / metabolism*

Substances

  • DNA, Fungal
  • RNA, Fungal
  • RNA, Transfer, Arg
  • RNA, Transfer, Glu
  • Saccharomyces cerevisiae Proteins
  • RNA, Transfer
  • TRM9 protein, S cerevisiae
  • tRNA Methyltransferases
  • Uridine