Bacterial expression, purification and preliminary X-ray crystallographic characterization of the invertase inhibitor Nt-CIF from tobacco

Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2279-82. doi: 10.1107/s0907444903021036. Epub 2003 Nov 27.

Abstract

Acid invertases catalyzing the breakdown of sucrose are regulated at the post-translational level by extracellular inhibitory proteins of 16-20 kDa molecular weight in a pH-dependent manner. Little is known about the characteristics of the underlying protein-protein interaction. Here, the expression, purification, characterization, crystallization and initial X-ray analysis of a biologically active invertase inhibitor Nt-CIF from tobacco is reported. Four crystal forms covering a wide pH range have been obtained and data sets at resolutions higher than 2.5 A have been collected.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular
  • Crystallography, X-Ray
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / metabolism
  • Enzyme Inhibitors / pharmacology
  • Gene Expression
  • Plant Proteins / biosynthesis*
  • Plant Proteins / chemistry*
  • Plant Proteins / genetics
  • Plant Proteins / pharmacology
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / pharmacology
  • Tobacco / chemistry*
  • beta-Fructofuranosidase / antagonists & inhibitors

Substances

  • Enzyme Inhibitors
  • INH protein, Nicotiana tabacum
  • Plant Proteins
  • Recombinant Fusion Proteins
  • beta-Fructofuranosidase