Structure and expression of the phosphoglycerate kinase (Pgk) gene of Drosophila melanogaster

Mol Gen Genet. 1992 Nov;235(2-3):213-20. doi: 10.1007/BF00279363.


The gene that encodes phosphoglycerate kinase in Drosophila melanogaster (Pgk) has been isolated and characterized. There is a single copy of Pgk in the Drosophila genome located at cytogenetic position 23A1-2. Transcripts of Pgk are 1.6 kb long and are found during development with a profile similar to the expression pattern of other genes of the glycolytic pathway. There are substantial amounts of maternal transcript in early embryos which decline in abundance until mid-embryogenesis when transcript levels increase; levels remain high, during larval stages, fall during pupariation and rise again at emergence. The nucleotide sequence of the Pgk gene reveals two small introns, one of which is at a position identical to the site of an intron found in Pgk genes from other organisms. The Pgk gene has no TATA box in the region of transcription initiation and has multiple transcription initiation sites that are closely spaced within 110 nucleotides of the translation start site.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • DNA / genetics
  • DNA / isolation & purification
  • DNA Probes
  • Drosophila melanogaster / enzymology*
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / physiology
  • Embryo, Nonmammalian / physiology
  • Genes
  • Humans
  • In Situ Hybridization
  • Introns
  • Molecular Sequence Data
  • Phosphoglycerate Kinase / genetics*
  • Protein Biosynthesis
  • RNA, Messenger / genetics
  • RNA, Messenger / isolation & purification
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Transcription, Genetic


  • DNA Probes
  • RNA, Messenger
  • DNA
  • Phosphoglycerate Kinase

Associated data

  • GENBANK/Z14029