Expression of the murine glutathione S-transferase alpha3 (GSTA3) subunit is markedly induced during adipocyte differentiation: activation of the GSTA3 gene promoter by the pro-adipogenic eicosanoid 15-deoxy-Delta12,14-prostaglandin J2

Biochem Biophys Res Commun. 2003 Dec 26;312(4):1226-35. doi: 10.1016/j.bbrc.2003.11.068.

Abstract

The expression of class alpha, micro, and pi glutathione S-transferases (GSTs) has been examined during the adipose conversion of mouse 3T3-L1 cells. The GSTA4, GSTM1, and GSTP1/2 subunits are expressed constitutively in confluent 3T3-L1 cells, and their levels remain essentially unchanged during adipocyte differentiation. By contrast, the GSTA3 subunit is virtually undetectable in confluent 3T3-L1 cells under basal conditions, but its expression is markedly induced during adipose conversion. Inhibition of the 3T3-L1 adipogenic program demonstrated that GSTA3 expression is associated specifically with acquisition of the adipocytic phenotype. Reporter gene assays demonstrated that the mouse GSTA3 5(')-upstream region is transcriptionally activated by 15-deoxy-Delta(12,14)-prostaglandin J(2) through an antioxidant response element, suggesting that this pro-adipogenic eicosanoid may be involved in regulating GSTA3 expression during adipogenesis. These data suggest a previously unrecognised role for GSTs in mouse adipocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / cytology*
  • Adipocytes / enzymology*
  • Animals
  • Cell Differentiation / physiology
  • Eicosanoids / metabolism
  • Gene Expression Regulation, Enzymologic / physiology*
  • Glutathione Transferase / genetics*
  • Glutathione Transferase / metabolism*
  • Mice
  • Promoter Regions, Genetic
  • Prostaglandin D2 / analogs & derivatives*
  • Prostaglandin D2 / genetics*
  • Prostaglandin D2 / metabolism*
  • Protein Subunits
  • Transcriptional Activation / physiology

Substances

  • 15-deoxy-delta(12,14)-prostaglandin J2
  • Eicosanoids
  • Protein Subunits
  • GSTA3 protein, mouse
  • Glutathione Transferase
  • Prostaglandin D2