In this paper, cold-water irrigation multiplication technique was used to solve the difficulty of multiplying rice TGMS line 96-5-2S(Oryza sative L.) with double low critical temperature values under natural air temperature condition. The results indicated that irrigating rice TGMS line 96-5-2S with different temperature underground cold water from the stage of pistil and stamen primordia differentiation to the stage of pollen filling for 15 days, and keeping the water depth 18-22 cm, the seed set rate of 96-5-2S decreased with increasing irrigating water temperature. When the average temperature of irrigation water was 18.5-19.8 degrees C, the seed set rate ranged from 40.5% to 57.6%, and the yield ranged from 3.30 t.hm-2 to 4.35 t.hm-2. While the temperature was 20.5-21.3 degrees C, the seed set rate and yield sharply decreased to 2.5-10.4% and 0.21-0.90 t.hm-2, respectively. When the average temperature was 22.3-23.5 degrees C, both the seed set rate and yield were zero. Under the same irrigation water temperature (average 19.8 degrees C) and the same irrigating period (phase IV to phase VII), the seed set rate and yield of 96-5-2S with deep water irrigation (18-22 cm) was very significantly higher than those of 96-5-2S with shallow water irrigation (7-10 cm). Under the conditions of same irrigation water temperature (average 19.8 degrees C) and same water depth (18-22 cm) but different irrigating periods (15 days from phase IV to phase VII, 20 days from phase III to phase VII, and 25 days from phase II to phase VII), the difference of seed set rate and yield of 96-5-2S between 15-day treatment and 20-25-day treatment was not significantly different. It is confident that 96-5-2S could multiply with underground cold water irrigation. The main technique index was that irrigating water temperature should be 18-20 degrees C, irrigating period should be from phase IV to phase VII, and irrigating water depth should be 18-22 cm.