The needle length of bacterial injectisomes is determined by a molecular ruler

Science. 2003 Dec 5;302(5651):1757-60. doi: 10.1126/science.1091422.

Abstract

Size determination represents a fundamental requirement for multicomponent biological structures. Some pathogenic bacteria possess a weapon derived from the flagellum. Like the flagellum, this type-III secretion apparatus, called the injectisome, has a transmembrane basal body, but the external component is a needle-like structure instead of a hook and a filament. Here, we provide evidence that the length of this needle is determined by the size of a protein, YscP, acting as a molecular ruler.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Bacterial Proteins / analysis
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / physiology*
  • Flagella / chemistry
  • Genes, Bacterial
  • Genetic Complementation Test
  • Microscopy, Electron
  • Mutation
  • Sequence Deletion
  • Yersinia enterocolitica / chemistry*
  • Yersinia enterocolitica / ultrastructure*

Substances

  • Bacterial Proteins
  • FliK protein, Bacteria
  • YscP protein, Yersinia enterocolitica