Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells

Nat Biotechnol. 2004 Jan;22(1):53-4. doi: 10.1038/nbt922. Epub 2003 Dec 7.


We have observed karyotypic changes involving the gain of chromosome 17q in three independent human embryonic stem (hES) cell lines on five independent occasions. A gain of chromosome 12 was seen occasionally. This implies that increased dosage of chromosome 17q and 12 gene(s) provides a selective advantage for the propagation of undifferentiated hES cells. These observations are instructive for the future application of hES cells in transplantation therapies in which the use of aneuploid cells could be detrimental.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aneuploidy
  • Animals
  • Cell Culture Techniques / methods
  • Cell Differentiation
  • Cells, Cultured
  • Chromosome Aberrations*
  • Chromosome Banding
  • Chromosomes, Human, Pair 12*
  • Chromosomes, Human, Pair 17*
  • Embryo, Mammalian / cytology*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Karyotyping
  • Models, Genetic
  • Specimen Handling
  • Stem Cells / cytology*
  • Time Factors